CF405S
CF®405S (Ex-Max 404 nm/Em-Max 431 nm)
CF®405S from Biotium is a fluorophore with an excitation peak at 404 nm and an emission peak at 431 nm. It is spectrally similar to Alexa Fluor™ 405. It can be use in fluorescence microscopy, specifically its been validated in Structured Illumination (SIM), and flow cytometry. However, in flow cytometry, superior alternatives are Brilliant™ Violet 421, SuperNova v428 and StarBright Violet 440.
Excitation & emission spectra
Key facts
- Excitation maximum
- 404 nm
- Emission maximum
- 431 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 33000
- Molecular weight
- 1169
- Commercial antibodies
- 4.4k from 1 supplier
CF405S has an excitation maximum at 404 nm and an emission maximum at 431 nm, a Stokes shift of 27 nm. Its extinction coefficient is 33000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is CF405S?
- CF®405S from Biotium is a fluorophore with an excitation peak at 404 nm and an emission peak at 431 nm. It is spectrally similar to Alexa Fluor™ 405. It can be use in fluorescence microscopy, specifically its been validated in Structured Illumination (SIM), and flow cytometry. However, in flow cytometry, superior alternatives are Brilliant™ Violet 421, SuperNova v428 and StarBright Violet 440.
- What laser excites CF405S?
- CF405S is optimally excited by the 405 laser line. Its excitation maximum is 404 nm.
- What filter should I use to detect CF405S?
- A 450/50 filter captures the peak of the CF405S emission near 431 nm with minimal spillover.
- How bright is CF405S?
- CF405S has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to CF405S?
- FluoroFinder tracks 4438 commercially available CF405S conjugates, searchable by target, clone, host and application.