DyLight 350
DyLight™ 350 (Ex-Max 353 nm/Em-Max 432 nm)
DyLight™ 350 has an excitation peak of 353 nm and an emission peak of 432 nm. It is spectrally similar to Alexa Fluor™ 350, iFluor® 350, AMCA, and Thioflavin T. DyLight™ 350 is most commonly used in flow cytometery and fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 353 nm
- Emission maximum
- 432 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
- Relative brightness
- 1 of 5
- Extinction coefficient
- 15000
- Commercial conjugates
- 1.1k from 8 suppliers
DyLight 350 has an excitation maximum at 353 nm and an emission maximum at 432 nm, a Stokes shift of 79 nm. Its extinction coefficient is 15000 M⁻¹cm⁻¹. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is DyLight 350?
- DyLight™ 350 has an excitation peak of 353 nm and an emission peak of 432 nm. It is spectrally similar to Alexa Fluor™ 350, iFluor® 350, AMCA, and Thioflavin T. DyLight™ 350 is most commonly used in flow cytometery and fluorescence microscopy applications.
- What laser excites DyLight 350?
- DyLight 350 is optimally excited by the 355 laser line. Its excitation maximum is 353 nm.
- What filter should I use to detect DyLight 350?
- A 450/50 filter captures the peak of the DyLight 350 emission near 432 nm with minimal spillover.
- How bright is DyLight 350?
- DyLight 350 has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to DyLight 350?
- FluoroFinder tracks 1113 commercially available DyLight 350 conjugates, searchable by target, clone, host and application.