Indo-Lo (Ca2+free)
Indo-Lo (Ca2+free) (Ex-Max 345 nm/Em-Max 484 nm)
Indo-1, AM is a ratiometric calcium indicator optimally excited by a 355nm UV laser or excitation source. After Indo-1, AM is loaded into live cells, it is hydrolyzed and available to bind cytoplasmic Ca2+. Probe bound to Ca2+ emit at ~400nm, while probe unbound to Ca2+ emits at ~475nm. Because of these unique spectral characteristics, it's the preferred calcium indicator for flow cytometry and is often frequently used in fluorescence live-cell microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 345 nm
- Emission maximum
- 484 nm
- Optimal laser line
- 355
- Optimal filter
- 450/50
Indo-Lo (Ca2+free) has an excitation maximum at 345 nm and an emission maximum at 484 nm, a Stokes shift of 139 nm. It is optimally excited by the 355 laser line.
Frequently asked questions
- What is Indo-Lo (Ca2+free)?
- Indo-1, AM is a ratiometric calcium indicator optimally excited by a 355nm UV laser or excitation source. After Indo-1, AM is loaded into live cells, it is hydrolyzed and available to bind cytoplasmic Ca2+. Probe bound to Ca2+ emit at ~400nm, while probe unbound to Ca2+ emits at ~475nm. Because of these unique spectral characteristics, it's the preferred calcium indicator for flow cytometry and is often frequently used in fluorescence live-cell microscopy.
- What laser excites Indo-Lo (Ca2+free)?
- Indo-Lo (Ca2+free) is optimally excited by the 355 laser line. Its excitation maximum is 345 nm.
- What filter should I use to detect Indo-Lo (Ca2+free)?
- A 450/50 filter captures the peak of the Indo-Lo (Ca2+free) emission near 484 nm with minimal spillover.