Alexa Fluor 405
Alexa Fluor™ 405 (Ex-Max 401 nm/Em-Max 421 nm)
Alexa Fluor™ 405 (AF405, Alexa 405) has an excitation peak at 401 nm and an emission peak at 421 nm and is similar in size, brightness and application to ABflo 405® (ABclonal) and Cascade Blue® (ThermoFisher Scientific). Alexa 405 is commonly used in flow cytometery and fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 401 nm
- Emission maximum
- 421 nm
- Optimal laser line
- 405
- Optimal filter
- 450/50
- Relative brightness
- 2 of 5
- Extinction coefficient
- 34000
- Molecular weight
- 1028
- Commercial antibodies
- 24k from 10 suppliers
Alexa Fluor 405 has an excitation maximum at 401 nm and an emission maximum at 421 nm, a Stokes shift of 20 nm. Its extinction coefficient is 34000 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Alexa Fluor 405?
- Alexa Fluor™ 405 (AF405, Alexa 405) has an excitation peak at 401 nm and an emission peak at 421 nm and is similar in size, brightness and application to ABflo 405® (ABclonal) and Cascade Blue® (ThermoFisher Scientific). Alexa 405 is commonly used in flow cytometery and fluorescence microscopy applications.
- What laser excites Alexa Fluor 405?
- Alexa Fluor 405 is optimally excited by the 405 laser line. Its excitation maximum is 401 nm.
- What filter should I use to detect Alexa Fluor 405?
- A 450/50 filter captures the peak of the Alexa Fluor 405 emission near 421 nm with minimal spillover.
- How bright is Alexa Fluor 405?
- Alexa Fluor 405 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to Alexa Fluor 405?
- FluoroFinder tracks 23978 commercially available Alexa Fluor 405 conjugates, searchable by target, clone, host and application.