EvaGreen
EvaGreen (Ex-Max 498 nm/Em-Max 533 nm)
EvaGreen from Biotium is a fluorogenic nucleic acid stain often used for in-gel detection of DNA and qPCR amplification. EvaGreen is compatible with qPCR, HRM and LAMP.
Excitation & emission spectra
Key facts
- Excitation maximum
- 498 nm
- Emission maximum
- 533 nm
- Optimal laser line
- 488
- Optimal filter
- 525/50
EvaGreen has an excitation maximum at 498 nm and an emission maximum at 533 nm, a Stokes shift of 35 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is EvaGreen?
- EvaGreen from Biotium is a fluorogenic nucleic acid stain often used for in-gel detection of DNA and qPCR amplification. EvaGreen is compatible with qPCR, HRM and LAMP.
- What laser excites EvaGreen?
- EvaGreen is optimally excited by the 488 laser line. Its excitation maximum is 498 nm.
- What filter should I use to detect EvaGreen?
- A 525/50 filter captures the peak of the EvaGreen emission near 533 nm with minimal spillover.