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Tri-Color

Tri-Color (Ex-Max 564 nm/Em-Max 670 nm)

Tri-Color is identical to the PE-Cy5/ PE-Cyanine 5 tandem fluorophore for use in flow cytometry.

Excitation & emission spectra

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Key facts

Excitation maximum
564 nm
Emission maximum
670 nm
Optimal laser line
488-561
Optimal filter
660/20
Relative brightness
2 of 5
Commercial conjugates
16 from 3 suppliers

Tri-Color has an excitation maximum at 564 nm and an emission maximum at 670 nm, a Stokes shift of 106 nm. It is optimally excited by the 488-561 laser line.

Frequently asked questions

What is Tri-Color?
Tri-Color is identical to the PE-Cy5/ PE-Cyanine 5 tandem fluorophore for use in flow cytometry.
What laser excites Tri-Color?
Tri-Color is optimally excited by the 488-561 laser line. Its excitation maximum is 564 nm.
What filter should I use to detect Tri-Color?
A 660/20 filter captures the peak of the Tri-Color emission near 670 nm with minimal spillover.
How bright is Tri-Color?
Tri-Color has a relative brightness of 2 out of 5.
How many antibodies are available conjugated to Tri-Color?
FluoroFinder tracks 16 commercially available Tri-Color conjugates, searchable by target, clone, host and application.