CellROX Orange
CellROX Orange (Ex-Max 545 nm/Em-Max 565 nm)
CellROX® Orange is a fluorogenic probe for measuring oxidative stress in live cells. This cell-permeant dye is non-fluorescent while in a reduced state and exhibits bright orange fluorescence upon oxidation by reactive oxygen species (ROS), with absorption/emission maxima of ~ 545/565 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 545 nm
- Emission maximum
- 565 nm
- Optimal laser line
- 532
- Optimal filter
- 575/36
CellROX Orange has an excitation maximum at 545 nm and an emission maximum at 565 nm, a Stokes shift of 20 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is CellROX Orange?
- CellROX® Orange is a fluorogenic probe for measuring oxidative stress in live cells. This cell-permeant dye is non-fluorescent while in a reduced state and exhibits bright orange fluorescence upon oxidation by reactive oxygen species (ROS), with absorption/emission maxima of ~ 545/565 nm.
- What laser excites CellROX Orange?
- CellROX Orange is optimally excited by the 532 laser line. Its excitation maximum is 545 nm.
- What filter should I use to detect CellROX Orange?
- A 575/36 filter captures the peak of the CellROX Orange emission near 565 nm with minimal spillover.