HiLyte 555
HiLyte™ 555 (Ex-Max 554 nm/Em-Max 570 nm)
HiLyte™ Fluor 555 from AnaSpec has an excitation peak at 555 nm and an emission peak at 570 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 554 nm
- Emission maximum
- 570 nm
- Optimal laser line
- 532
- Optimal filter
- 586/25
- Relative brightness
- 2 of 5
- Extinction coefficient
- 150000
- Quantum yield
- 0.1
HiLyte 555 has an excitation maximum at 554 nm and an emission maximum at 570 nm, a Stokes shift of 16 nm. Its extinction coefficient is 150000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is HiLyte 555?
- HiLyte™ Fluor 555 from AnaSpec has an excitation peak at 555 nm and an emission peak at 570 nm.
- What laser excites HiLyte 555?
- HiLyte 555 is optimally excited by the 532 laser line. Its excitation maximum is 554 nm.
- What filter should I use to detect HiLyte 555?
- A 586/25 filter captures the peak of the HiLyte 555 emission near 570 nm with minimal spillover.
- How bright is HiLyte 555?
- HiLyte 555 has a relative brightness of 2 out of 5 (quantum yield 0.1).