355

Laser Line

379/28

Common Filter

348

Excitation Max

395

Emission Max

835

Antibodies


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Dyes similar to BUV395 based on emission

Indo-Hi (Ca2+bound) Spark UV 387 StarBright UltraViolet 400

About BUV395


Brilliant UltraViolet™ 395 (BUV395) is a UV-emitting base polymer dye that can be excited by the 355 nm ultraviolet laser and collected using a 379/28 bandpass filter. BUV935 has an excitation peak at 348 nm and an emission peak at 395. BUV395's fairly unique excitation and emission spectrum causes relatively little spillover into other common detectors and conflicts with very few other commonly used fluorophores. BUV395 has a medium level of brightness and is said to be as bright as or brighter than similar FITC reagents. This dye is part of the Brilliant UltraViolet dye family, developed and sold by BD Biosciences. These dyes were optimized for use with multicolor flow cytometry. BUV395 forms the backbone of the family, as all other members are tandems that use it as the acceptor, thus making them excitable by the same 355 nm UV laser.


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