405

Laser Line

780/60

Common Filter

405

Excitation Max

750

Emission Max

729

Antibodies


Spectra Viewer

Dyes similar to Brilliant Violet 750 based on emission

BB755-P Live-or-Dye 615/740 MitoView 720 StarBright Violet 760 Zombie NIR

About Brilliant Violet 750


Brilliant Violet™ 750 (BV750) is a red-emitting tandem fluorophore that can be excited by the 405 nm violet laser and collected using a 780/60 bandpass filter with a 740 LP dichroic mirror. BV750 has an excitation peak at 405 nm and an emission peak at 750 nm. Due to its unique stoke’s shift, no dyes have identical or near identical excitation and emission spectrums. BV750 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large stoke’s shift, however BV750 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant Violet dye line. The Brilliant Violet dye line itself is very popular because other dyes in the line also use the Brilliant Violet 421 core as the donor molecule, while varied acceptor molecules emit light at various points across the visible light spectrum. This allows for multiple dyes to be compatible while excited by the single laser. In cytometry, a user may populate all the available channels off their 405-violet laser with Brilliant Violet dyes.


Popular Brilliant Violet 750 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
100249
FC CD3
Mouse
Rat
IgG2b κ
17A2
50 μg
$275
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BioLegend
100467
FC CD4
Mouse
Rat
IgG2b κ
GK1.5
50 μg
$286
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BioLegend
344643
FC CD4
Human
Mouse
IgG1 κ
SK3
25 Tests
$209
View
BioLegend
362555
FC CD56
Human
Mouse
IgG1 κ
5.1H11
25 Tests
$209
View
BioLegend
302849
FC CD27
Human,African Green Monkey,Baboon,Cynomolgus,Rhesus,Squirrel Monkey
Mouse
IgG1 κ
O323
25 Tests
$209
View
BD Biosciences
747111
FC CD185
Human
Rat
IgG2b κ
RF8B2
50 μg
$495
View
BD Biosciences
747316
FC Siglec-F
Mouse
Rat
IgG2a κ
E50-2440
50 μg
$350
View
BioLegend
506358
ICS TNF alpha
Mouse
Rat
IgG1 κ
MP6-XT22
50 μg
$325
View
BioLegend
117357
FC CD11c
Mouse
Armenian Hamster
IgG
N418
50 μg
$286
View
BD Biosciences
751830
FC CD123
Human
Mouse
IgG1 κ
6H6
50 μg
$495
View
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