About Brilliant Violet 750

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Brilliant Violet™ 750 (BV750) is a red-emitting tandem fluorophore that can be excited by the 405 nm violet laser and collected using a 780/60 bandpass filter with a 740 LP dichroic mirror. BV750 has an excitation peak at 405 nm and an emission peak at 750 nm. Due to its unique stoke’s shift, no dyes have identical or near identical excitation and emission spectrums. BV750 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large stoke’s shift, however BV750 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant Violet dye line. The Brilliant Violet dye line itself is very popular because other dyes in the line also use the Brilliant Violet 421 core as the donor molecule, while varied acceptor molecules emit light at various points across the visible light spectrum. This allows for multiple dyes to be compatible while excited by the single laser. In cytometry, a user may populate all the available channels off their 405-violet laser with Brilliant Violet dyes.

Dyes similar to Brilliant Violet 750 based on emission

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Brilliant Blue 755-P Live-or-Dye 615/740 MitoView 720 StarBright Violet 760 Zombie NIR