Brilliant Violet 750
Brilliant™ Violet 750 (Ex-Max 405 nm/Em-Max 750 nm)
Brilliant™ Violet 750 (BV750) is a red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 780/60 bandpass filter with a 740 LP dichroic mirror. BV750 has an excitation peak at 405 nm and an emission peak at 750 nm. Due to its unique Stokes shift, no dyes have identical or near identical excitation and emission spectrums. BV750 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large Stokes shift, however BV750 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Excitation & emission spectra
Key facts
- Excitation maximum
- 405 nm
- Emission maximum
- 750 nm
- Optimal laser line
- 405
- Optimal filter
- 780/60
- Relative brightness
- 3 of 5
- Extinction coefficient
- 2301131
- Quantum yield
- 0.057
- Molecular weight
- 70000
- Commercial conjugates
- 73 from 3 suppliers
Brilliant Violet 750 has an excitation maximum at 405 nm and an emission maximum at 750 nm, a Stokes shift of 345 nm. Its extinction coefficient is 2301131 M⁻¹cm⁻¹. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is Brilliant Violet 750?
- Brilliant™ Violet 750 (BV750) is a red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 780/60 bandpass filter with a 740 LP dichroic mirror. BV750 has an excitation peak at 405 nm and an emission peak at 750 nm. Due to its unique Stokes shift, no dyes have identical or near identical excitation and emission spectrums. BV750 exhibits a medium level of brightness and is most often used in flow cytometry. Older instruments may not be set up for a dye with such a large Stokes shift, however BV750 is especially well suited for spectral cytometers or sorters. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
- What laser excites Brilliant Violet 750?
- Brilliant Violet 750 is optimally excited by the 405 laser line. Its excitation maximum is 405 nm.
- What filter should I use to detect Brilliant Violet 750?
- A 780/60 filter captures the peak of the Brilliant Violet 750 emission near 750 nm with minimal spillover.
- How bright is Brilliant Violet 750?
- Brilliant Violet 750 has a relative brightness of 3 out of 5 (quantum yield 0.057).
- How many antibodies are available conjugated to Brilliant Violet 750?
- FluoroFinder tracks 73 commercially available Brilliant Violet 750 conjugates, searchable by target, clone, host and application.