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Zombie NIR
Zombie NIR™ (Ex-Max 715 nm/Em-Max 743 nm)
Zombie NIR™ from Biolegend is an amine-reactive viability dye useful in flow cytometry when fixation and permeabilization of the cells is required prior to analysis. It has an excitation peak at 715 nm and an emission peak at 743 nm, however in flow cytometry it is recommended for excitation with the red 640 nm laser.
Excitation & emission spectra
Key facts
- Excitation maximum
- 715 nm
- Emission maximum
- 743 nm
- Optimal laser line
- 628-640
- Optimal filter
- 710/50
- Commercial antibodies
- 4 from 2 suppliers
Zombie NIR has an excitation maximum at 715 nm and an emission maximum at 743 nm, a Stokes shift of 28 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Zombie NIR?
- Zombie NIR™ from Biolegend is an amine-reactive viability dye useful in flow cytometry when fixation and permeabilization of the cells is required prior to analysis. It has an excitation peak at 715 nm and an emission peak at 743 nm, however in flow cytometry it is recommended for excitation with the red 640 nm laser.
- What laser excites Zombie NIR?
- Zombie NIR is optimally excited by the 628-640 laser line. Its excitation maximum is 715 nm.
- What filter should I use to detect Zombie NIR?
- A 710/50 filter captures the peak of the Zombie NIR emission near 743 nm with minimal spillover.
- How many antibodies are available conjugated to Zombie NIR?
- FluoroFinder tracks 4 commercially available Zombie NIR conjugates, searchable by target, clone, host and application.