University of Wisconsin - Madison
UWCCC Flow Cytometry Laboratory

Design a panel and find antibodies today at

https://app.fluorofinder.com/uwflow
Logo: FluoroFinder

Attune-WIMR & RASR Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

440 / 50

417

 Alexa Fluor 405
Brilliant Violet 421
Cascade Blue
DyLight 405
eBFP
eFluor 450
Pacific Blue
SuperBright 436
V450
Vio Blue

512 / 25

495

 Alexa Fluor 430
AmCyan
Brilliant Violet 510
Cascade Yellow
Krome Orange
Pacific Green
Pacific Orange
V500
Vio Green
CF405L

603 / 48

555

 Brilliant Violet 605
eVolve 605
Qdot 605
SuperBright 600
DyLight 405LS
eFluor 605NC

710 / 50

650

 Qdot 705
SuperBright 702
Laser

488

530 / 30

503

 Alexa Fluor 488
Brilliant Blue 515
Vio Bright 515
Vio Bright FITC
YFP
ATTO 488
CF488A
CF503R
Cy2
DyLight 488

590 / 40

555

 Live/Dead Fix Red
PE-Vio615
Propidium Iodide
Qdot 585
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Texas Red

695 / 40

650

 PerCP
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
Brilliant Blue 700
PE-Cy5
PE-Cy5.5
Laser

561

585 / 16

577

 PE
Propidium Iodide
tdTomato
CF550R
CF568
CF570

620 / 15

600

 dsRed
DyLight 594
Propidium Iodide
ECD
PE-CF594
PE-Dazzle 594
PE-Texas Red

695 / 40

650

 PE-Cy5.5
7-AAD

780 / 60

740

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

633

670 / 40

654

 APC
DyLight 650
Live/Dead Fix Far Red
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

720 / 30

690

 Alexa Fluor 700
APC-Alexa 700
APC-R700

780 / 60

740

 APC-Alexa 750
APC-Cy7
APC-Vio770
APC-eFluor 780
APC-Fire 750
APC-H7

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.