University of Wisconsin - Madison
UWCCC Flow Cytometry Laboratory

Design a panel and find antibodies today at

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ImageStreamX Mark II-WIMR Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

488

533 / 55

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Alexa Fluor 488
FITC
GFP
LysoTracker Green
MitoTracker Green
PKH67
Spectrum Green
YFP
ATTO 488
Brilliant Blue 515

577 / 35

--

CellTracker Orange
Cy3
dsRed
mOrange
PE
PKH26
Sytox Orange
Alexa Fluor 555
Cyanine 3 SE

610 / 30

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ECD
eFluor 625NC
PE-Alexa 610
PE-CF594
PE-Texas Red
Propidium Iodide
Qdot 625
RFP
PE-Dazzle 594
PE-eFluor 610

702 / 85

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Cy5.5
DRAQ 5
eFluor 650NC
Fura Red AM (Ca2+free)
PE-Alexa 647
PE-Cy5
PerCP
PerCP-Cy5.5
Qdot 705
7-AAD

762 / 35

--

PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

642

702 / 85

--

Alexa Fluor 647
Alexa Fluor 660
Alexa Fluor 680
Alexa Fluor 700
APC
Cy5
DRAQ 5
DyLight 649
DyLight 680
PE-Alexa 647
PE-Cy5
PerCP
PerCP-Cy5.5

762 / 35

--

APC-Alexa 750
APC-Cy7
APC-H7
DyLight 750
PE-Alexa 750
PE-Cy7
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.