University of Wisconsin - Madison
UWCCC Flow Cytometry Laboratory

Design a panel and find antibodies today at

https://app.fluorofinder.com/uwflow
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Attune V6 - BioTech Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 40

417

 Alexa Fluor 405
BFP
Brilliant Violet 421
CellTrace Violet
CFP
DAPI
mTurquoise
Vio Blue
DyLight 405
Pacific Blue

525 / 50

495

 Brilliant Violet 510
Pacific Green

610 / 20

555

 Brilliant Violet 605
SuperBright 600

660 / 20

635

 Brilliant Violet 650
SuperBright 645

710 / 50

680

 Brilliant Violet 711
SuperBright 702

780 / 60

740

 Brilliant Violet 785
Brilliant Violet 786
Laser

488

530 / 30

503

 Alexa Fluor 488
Brilliant Blue 515
FITC
GFP
DyLight 488

695 / 40

555

 Brilliant Blue 700
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
7-AAD
PE-Cy5
PE-Cy5.5
Laser

561

585 / 16

577

 Alexa Fluor 555
mOrange
PE
RFP
tdTomato
Cy3

620 / 15

600

 Alexa Fluor 594
dsRed
mCherry
PE-CF594
PE-Dazzle 594
PE-Texas Red
DyLight 594

780 / 60

650

 PE-Cy7
PE-Vio770
Laser

633

670 / 14

654

 Alexa Fluor 647
APC
Cy5

720 / 30

690

 Alexa Fluor 680
Alexa Fluor 700
APC-Alexa 700
APC-R700
APC-Cy5.5

780 / 60

740

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Vio770
APC-Fire 750

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.