University of Wisconsin - Madison
UWCCC Flow Cytometry Laboratory

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Aria-Jayne - BioTech Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 Alexa Fluor 350
Brilliant Ultraviolet 395
CF350

530 / 30

505

 Brilliant Ultraviolet 496
Brilliant Ultraviolet 563

670 / 25

630

 Brilliant Ultraviolet 661
Brilliant Ultraviolet 737
Brilliant Ultraviolet 805
Laser

405

450 / 40

--

 Alexa Fluor 405
Brilliant Violet 421
Pacific Blue
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

510 / 50

502

 Alexa Fluor 430
Brilliant Violet 510
Pacific Orange
CF405L
CF430
CF440
DyLight 405LS
Krome Orange
V500
Vio Green

610 / 20

595

 Brilliant Violet 605
eFluor 625NC
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 25

630

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

690

 Brilliant Violet 650
Brilliant Violet 711
Brilliant Violet 750
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

502

 Alexa Fluor 488
Alexa Fluor 514
Brilliant Blue 515
DyLight 488
FITC
GFP
ATTO 488
CF488A
CF503R
Cy2

695 / 40

655

 Brilliant Blue 700
PerCP
PerCP-Cy5
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
Laser

561

582 / 15

--

 Alexa Fluor 532
Alexa Fluor 546
Alexa Fluor 555
Cy3
mOrange
PE
ATTO 550
CF550R
CF568
CF570

610 / 20

600

 Alexa Fluor 488
mCherry
mStrawberry
PE-CF594
PE-eFluor 610
PE-Texas Red
CF583
CF583R
DyLight 594
ECD

670 / 14

630

 PE-Cy5
7-AAD

780 / 60

735

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
Alexa Fluor 660
APC
Cy5
CF640R
CF647
Cyanine 5 SE
DyLight 633

730 / 45

690

 Alexa Fluor 680
Alexa Fluor 700
APC-Alexa 700
APC-Cy5.5
APC-R700
redFluor 710
DyLight 680

780 / 60

755

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.