University of Wisconsin - Madison
UWCCC Flow Cytometry Laboratory

Design a panel and find antibodies today at

https://app.fluorofinder.com/uwflow
Logo: FluoroFinder

Fortessa-WIMR Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395

450 / 50

410

 Alexa Fluor 350
BFP
CMA-Chromomycin A3
DAPI
DyLight 350
FxCycle Violet
Hoechst (33258)
Hoechst (33342)
Indo-Hi (Ca2+bound)
Live/Dead Fix Blue
Sytox Blue

515 / 30

505

 Brilliant Ultraviolet 496
Brilliant Ultraviolet 737
Brilliant Ultraviolet 805
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
CellTracker Blue
CFP
DAPI
DyLight 405
eFluor 450
Hoechst (33258)
Hoechst (33342)
Live/Dead Fix Violet
Pacific Blue
V450

525 / 50

495

 Alexa Fluor 430
AmCyan
Brilliant Violet 510
Cascade Yellow
Krome Orange
Live/Dead Fix Aqua
Pacific Orange
V500
CF405L
CF430

610 / 20

595

 Brilliant Violet 605
eFluor 605NC
eFluor 625NC
Live/Dead Fix Yellow
DyLight 405LS
eVolve 605
Qdot 605
SuperBright 600

670 / 30

635

 Brilliant Violet 650
Brilliant Violet 711
eFluor 650NC
eVolve 655
Qdot 655
Qdot 705
SuperBright 645
SuperBright 702

780 / 60

750

 Brilliant Violet 785
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
Alexa Fluor 514
Brilliant Blue 515
CFSE
Cy2
DyLight 488
Emerald GFP
FITC
GFP
Live/Dead Fix Green
Oregon Green 488
Pyronin Y
Rhodamine 110
Sytox Green
TO-Pro 1
TOTO-1
Vybrant DyeCycle Green
YFP

710 / 40

685

 PerCP
PerCP-Cy5
PerCP-Cy5.5
PerCP-eFluor 710
Propidium Iodide
7-AAD
Brilliant Blue 700
PerCP-Vio700
Laser

561

586 / 14

--

 Alexa Fluor 532
Alexa Fluor 546
Alexa Fluor 555
Cy3
DCF
dsRed
Live/Dead Fix Red
mBanana
mOrange
PE
Propidium Iodide
tdTomato

610 / 20

595

 Alexa Fluor 568
Alexa Fluor 594
Alexa Fluor 610
Cy3.5
DyLight 594
Live/Dead Fix Red
mCherry
MitoTracker Red
mKate2
mStrawberry
mTangerine
PE-Alexa 610
PE-CF594
PE-eFluor 610
PE-Texas Red
Propidium Iodide
RFP
Rhodamine Red-X

670 / 30

635

 mPlum
mRaspberry
PE-Alexa 647
PE-Cy5
Propidium Iodide
Sytox Orange
7-AAD

710 / 50

685

 PE-Cy5.5

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

643

670 / 14

--

 Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
Alexa Fluor 660
APC
Cy5
DRAQ 5
DyLight 633
DyLight 649
eFluor 660
FxCycle Far Red
Live/Dead Fix Red

730 / 44

710

 Alexa Fluor 680
Alexa Fluor 700
APC-Alexa 700
APC-Cy5.5
APC-R700
redFluor 710

780 / 60

750

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.