University of Washington
Cell Analysis Flow and Imaging Cytometry Core Lab

Design a panel and find antibodies today at

https://app.fluorofinder.com/uwcaf
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Fortessa Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395

740 / 35

685

 Brilliant Ultraviolet 737
Laser

405

450 / 50

--

 Brilliant Violet 421
DAPI
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

510 / 20

495

 Brilliant Violet 510
V500
Alexa Fluor 430
CF405L
CF430
CF440
Vio Green

575 / 25

550

 Brilliant Violet 570
DyLight 405LS
Qdot 565
Qdot 585

610 / 20

590

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

660 / 40

635

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 40

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

770

 Brilliant Violet 785
Qdot 800
Laser

488

525 / 50

505

 Alexa Fluor 488
eGFP
FITC
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

710 / 50

685

 PerCP
PerCP-Cy5.5
Propidium Iodide
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

532

575 / 26

--

 dsRed
PE
CF550R
CF570

610 / 20

600

 ECD
mCherry
PE-Texas Red
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

660 / 40

640

 PE-Cy5

780 / 40

740

 PE-Cy7
PE-Vio770
Laser

628

660 / 20

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
Cy5
DyLight 633
DyLight 650
NL 637

710 / 50

685

 Alexa Fluor 700
APC-R700

780 / 60

740

 APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.