University of Washington
Cell Analysis Flow and Imaging Cytometry Core Lab

Design a panel and find antibodies today at

https://app.fluorofinder.com/uwcaf
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ImageStreamX Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

457 / 45

--

 Alexa Fluor 405
Brilliant Violet 421
Cascade Blue
CFP
DAPI
DyLight 405
eFluor 450
Live/Dead Fix Violet
Pacific Blue
CF405M

533 / 55

--

 Alexa Fluor 430
Brilliant Violet 510
Cascade Yellow
Pacific Orange
Qdot 525
Brilliant Violet 570
CF405L
CF430
CF440
DyLight 405LS

577 / 35

--

 Qdot 565
Qdot 585
Brilliant Violet 570

610 / 30

--

 Brilliant Violet 605
eFluor 625NC
Qdot 625
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

702 / 85

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

762 / 35

--

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

533 / 55

--

 Alexa Fluor 488
DyLight 488
FITC
GFP
LysoTracker Green
MitoTracker Green
PKH67
Spectrum Green
YFP
ATTO 488

577 / 35

--

 Cy3
dsRed
mOrange
PE
PKH26
Sytox Orange
Alexa Fluor 555
Cyanine 3 SE

610 / 30

--

 ECD
PE-Alexa 610
PE-Texas Red
Propidium Iodide
Qdot 625
RFP
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

702 / 85

--

 DRAQ 5
Fura Red AM (Ca2+free)
PE-Alexa 647
PE-Cy5
PerCP
PerCP-Cy5.5
Qdot 705
7-AAD
Brilliant Blue 700
PerCP-eFluor 710

762 / 35

--

 PE-Alexa 750
PE-Cy7
Qdot 800
PE-Vio770
Laser

647

702 / 85

--

 Alexa Fluor 647
Alexa Fluor 660
Alexa Fluor 680
APC
Cy5
DRAQ 5
DyLight 649
DyLight 680
PE-Alexa 647
PE-Cy5
PerCP
PerCP-Cy5.5

762 / 35

--

 Alexa Fluor 750
APC-Alexa 750
APC-Cy7
APC-H7
Cy7
DyLight 750
PE-Alexa 750
PE-Cy7
APC-eFluor 780
APC-Fire 750

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.