University of Washington
Cell Analysis Flow and Imaging Cytometry Core Lab

Design a panel and find antibodies today at

https://app.fluorofinder.com/uwcaf
Logo: FluoroFinder

Canto RUO Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Brilliant Violet 421
eFluor 450
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
SuperBright 436
Vio Blue

525 / 50

505

 AmCyan
Brilliant Violet 510
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

605 / 40

595

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600
Laser

488

530 / 30

505

 Alexa Fluor 488
eGFP
FITC
Rhodamine 123
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

695 / 40

635

 PerCP
PerCP-Cy5.5
Propidium Iodide
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

586 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

600

 mCherry
PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 30

635

 PE-Cy5

710 / 50

685

 PE-Cy5.5

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

633

670 / 30

635

 Alexa Fluor 647
APC
eFluor 660
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650

730 / 45

690

 Alexa Fluor 700
APC-R700

780 / 60

750

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.