University of Washington
Cell Analysis Flow and Imaging Cytometry Core Lab

Design a panel and find antibodies today at

https://app.fluorofinder.com/uwcaf
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Canto 2 (3-laser) Configuration

Laser (nm) BP SP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

--

 Brilliant Violet 421
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
Vio Blue

510 / 50

--

502

 V500
Alexa Fluor 430
Brilliant Violet 510
DyLight 405LS
Vio Green
Laser

488

530 / 30

--

502

 Alexa Fluor 488
eGFP
FITC
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

585 / 42

--

556

 dsRed
PE

--

735

670

 PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

--

735

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

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--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
NL 637

780 / 60

--

735

 APC-Alexa 750
APC-Cy7
APC-H7
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.