University of Washington
Cell Analysis Flow and Imaging Cytometry Core Lab

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Aria 3 - Rm E386-A Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Brilliant Violet 421
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

525 / 50

505

 Brilliant Violet 510
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

605 / 40

595

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

660 / 20

630

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

705 / 70

670

 Brilliant Violet 711
Qdot 705
SuperBright 702
Laser

488

530 / 30

505

 Alexa Fluor 488
eGFP
FITC
Rhodamine 123
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

685 / 35

670

 PerCP
PerCP-Cy5.5
Propidium Iodide
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

582 / 15

570

 dsRed
PE
CF550R
CF568
CF570

610 / 20

600

 mCherry
PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

660 / 20

635

 PE-Cy5

695 / 40

685

 PE-Cy5.5

780 / 60

755

 PE-Cy7
PE-Vio770
Laser

633

670 / 30

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
NL 637

730 / 45

690

 Alexa Fluor 680
APC-Cy5.5
DyLight 680

780 / 60

755

 APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.