FACS Canto II Configuration

	Laser (nm)	LP	Optimal Colors Additional optimal fluorochrome options available on FluoroFinder.com.
Laser	405	450 / 50	--	Alexa Fluor 405 ATTO 425 Brilliant Violet 421 Brilliant Violet 480 CF405M CF405S cFluor V420 cFluor V450 CoraLite Plus 405 CytoCalcein Violet 450 CytoCalcein Violet 500 DY-415 DyLight 405 eFluor 450 ExoBrite 410/450 EV Membrane Stain HiLyte 405 iFluor 405 LysoBrite Blue LysoView 405 MaxLight 405 MemBrite Fix 405/430 mFluor Violet 450 MitoLite Blue FX490 MitoView 405 Nuclear Violet Nuclear Yellow Oxazole Blue Homodimer Pacific Blue POPO-1 PromoFluor 405 Spark Violet 423 Spectrum Aqua StarBright Violet 440 StarBright Violet 475 SuperBright 436 SuperNova v428 V450 Vio Blue Vio Bright V423 violetFluor 450
Laser	405	510 / 50	502	ATTO 425 ATTO 430LS Brilliant Violet 480 Brilliant Violet 510 CytoCalcein Violet 500 DY-395XL LipidSpot 488 LysoBrite Blue LysoSensor Yellow/Blue pH3.0 MitoLite Blue FX490 MitoLite Orange EX405 Nuclear Yellow Spark Violet 538 Spectrum Aqua StarBright Violet 475 StarBright Violet 515 Vio Green

Laser	488	530 / 30	502	Adirondack Green 520 Alexa Fluor 488 Alpha Fluor 488 ATTO 488 BactoView Live Green BCECF AM Brilliant Blue 515 CellTrace Oregon Green CF488A CF500 cFluor B515 cFluor B532 cFluor B548 Chromeo 488 Chromeo 505 CoraLite 532 CoraLite Plus 488 CytoTrace Green CMFDA DAF-FM DMAO DyLight 488 ExoBrite 490/515 EV Membrane Stain FITC FITC Plus HiLyte 488 iFluor 488 KIRAVIA Blue 520 LysoBrite Green LysoView 488 MaxLight 490 MemBrite Fix 488/515 MitoLite Green EX488 MitoLite Green FM MitoMark Green MitoROS 520 NBD Neuro DiO NL 493 NovaFluor Blue 530 Nuclear Green Nuclear Orange NucSpot 470 NucSpot Live 488 Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue ROS-490, Total Oxidative Stress Indicator PicoGreen PromoFluor 488P RealBlue 545 Spark Blue 550 Spark Blue 574 Spectrum Green SureLight 488 Syto 16 Syto 24 Thiazole Orange Homodimer Thiazole Orange Iodide Vio 488 Vio 515 Vio Bright 515 Vio Bright FITC VRDye 490
		585 / 42	556	Alexa Fluor 555 cFluor B548 cFluor BYG575 cFluor BYG610 Chromeo 494 CoraLite 532 Di-8-ANEPPS NBD NovaFluor Blue 610 / 30S Nuclear Green Nuclear Orange NucSpot 470 PE PE-CF594 PE-Dazzle 594 PE-DyLight 594 PE-Dyomics 590 PE-eFluor 610 PE-Vio615 PhenoVue 512 Nucleic Acid Stain RealBlue 545 SNARF 1 pH6.0 Spark Blue 574 StarBright Blue 580 StarBright Blue 615 SureLight PE-594 Thiazole Orange Homodimer Thiazole Orange Iodide
		--	610	ATTO 490LS Brilliant Blue 700 cFluor B690 cFluor BYG610 cFluor BYG667 cFluor BYG680 cFluor BYG710 cFluor BYG781 Chromeo 494 Di-8-ANEPPS NovaFluor Blue 610 / 30S NovaFluor Blue 660 / 40S NovaFluor Blue 690 PE-Alexa 610 PE-ATTO 594 PE-CF594 PE-Cy5 PE-Cy5.5 PE-Cy7 PE-Dazzle 594 PE-DyLight 594 PE-Dyomics 590 PE-Dyomics 647 PE-eFluor 610 PE-Fire 640 PE-Fire 700 PE-Fire 810 PerCP-eFluor 710 PerCP-Fire 806 PerCP-Vio700 PE-Vio615 PE-Vio670 PE-Vio770 PhenoVue 512 Nucleic Acid Stain RealBlue 705 RealBlue 744 RealBlue 780 RPE-CF647T Seta-R-PE-775 SNARF 1 pH6.0 StarBright Blue 615 StarBright Blue 675 StarBright Blue 700 StarBright Blue 765 StarBright Blue 810 SureLight PE-594 SureLight PE Cyanine 5.0 SureLight PE Cyanine 5.5 SureLight PE Cyanine 7.0
		--	670	ATTO 490LS Brilliant Blue 700 cFluor B690 cFluor BYG667 cFluor BYG680 cFluor BYG710 cFluor BYG781 Chromeo 494 Di-8-ANEPPS NovaFluor Blue 660 / 40S NovaFluor Blue 690 PE-Cy5 PE-Cy5.5 PE-Cy7 PE-Dyomics 647 PE-Fire 700 PE-Fire 810 PerCP-eFluor 710 PerCP-Fire 806 PerCP-Vio700 PE-Vio670 PE-Vio770 RealBlue 705 RealBlue 744 RealBlue 780 RPE-CF647T Seta-R-PE-775 StarBright Blue 675 StarBright Blue 700 StarBright Blue 765 StarBright Blue 810 SureLight PE Cyanine 5.0 SureLight PE Cyanine 5.5 SureLight PE Cyanine 7.0
		780 / 60	735	cFluor BYG781 PE-Cy7 PE-Fire 810 PerCP-Fire 806 PE-Vio770 RealBlue 744 RealBlue 780 Seta-R-PE-775 StarBright Blue 765 StarBright Blue 810 SureLight PE Cyanine 7.0

Laser	633	660 / 20	--	Alexa Fluor 633 Alexa Fluor 635 Alexa Fluor 647 Alexa Fluor 660 APC ATTO 620 ATTO 633 ATTO 647 ATTO 647N ATTO 655 Calcein Deep Red AM CellTracker Deep Red CF633 CF640R CF647 cFluor R685 Chromeo 642 CoraLite 647A CoraLite Plus 647 Cy5 CypHer5E DRAQ 9 DyLight 633 DyLight 649 DyLight 650 ExoBrite 640/660 EV Membrane Stain FluoroProbes647h HiLyte 647 iFluor 633 IRDye 650 Janelia Fluor 635 Janelia Fluor 646 LipidSpot 610 LysoBrite NIR LysoView 640 MaxLight 650 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoLite Deep Red FX660 MitoLite NIR FX690 MitoView 650 MitoView Fix 640 NL 637 NucSpot 640 NucSpot Far-Red Oyster 650 PhenoVue 641 Mitochondrial Stain PhenoVue Fluor 647 PhenoVue ROS-670, Total Oxidative Stress Indicator STAR 635 STAR 635P STAR RED SureLight APC SureLight P3 SureLight RPC Thiazole Red Thiazole Red Homodimer Vio 667 Vio Bright 667 Vio Bright R667 Vio R667
		--	685	Alexa Fluor 647 Alexa Fluor 660 APC-Alexa 680 APC-Alexa 700 APC-CF750 APC-CF770 APC-eFluor 780 APC-Fire 750 APC-Fire 810 APC-H7 APC-R700 APC-Vio770 ATTO 633 ATTO 647 ATTO 647N ATTO 655 CellTracker Deep Red cFluor R685 CoraLite Plus 647 CypHer5E DRAQ 9 DyLight 649 FluoroProbes647h HiLyte 647 IRDye 650 IRDye 680RD LipidSpot 610 LysoView 640 LysoView 680 MaxLight 650 MitoLite NIR FX690 MitoView 650 MitoView Fix 640 NovaFluor Red 725 NovaFluor Red 755 NucSpot 640 NucSpot 680 NucSpot Far-Red Oyster 650 PE-Fire 780 PhenoVue Fluor 647 PhenoVue ROS-670, Total Oxidative Stress Indicator Red 718 Seta-APC-705 STAR RED SureLight P3 Vio 667 Vio Bright 667 Vio Bright R667 Vio Bright R720 Vio R667 Vio R720
		780 / 60	735	APC-CF750 APC-CF770 APC-eFluor 780 APC-Fire 750 APC-Fire 810 APC-H7 APC-Vio770 DRAQ 9 LysoView 680 NovaFluor Red 755 PE-Fire 780

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.

University of Washington Pathology Flow Cytometry Core Facility

FACS Canto II Configuration

405

450 / 50

--

510 / 50

502

488

530 / 30

502

585 / 42

556

--

610

--

670

780 / 60

735

633

660 / 20

--

--

685

780 / 60

735

University of Washington
Pathology Flow Cytometry Core Facility