University of Washington
Pathology Flow Cytometry Core Facility

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Canto II - Disabled 19MAR24 Configuration

Laser (nm) BP SP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

--

 Alexa Fluor 405
Brilliant Violet 421
Brilliant Violet 480
DAPI
Pacific Blue
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450

510 / 50

--

502

 Brilliant Violet 510
CFP
Pacific Orange
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Krome Orange
V500
Laser

488

--

610

--

 Adirondack Green 520
Alexa Fluor 488
Alexa Fluor 555
Alpha Fluor 488
ATTO 488
BactoView Live Green
BCECF AM
Brilliant Blue 515
CellTrace Oregon Green
CF488A
CF500
cFluor B515
cFluor B532
cFluor B548
cFluor BYG575
cFluor BYG610
Chromeo 488
Chromeo 494
Chromeo 505
CoraLite 532
CoraLite Plus 488
CytoTrace Green CMFDA
DAF-FM
Di-8-ANEPPS
DMAO
DyLight 488
ExoBrite 490/515 EV Membrane Stain
FITC
FITC Plus
HiLyte 488
iFluor 488
KIRAVIA Blue 520
LysoBrite Green
LysoView 488
MaxLight 490
MemBrite Fix 488/515
MitoLite Green EX488
MitoLite Green FM
MitoMark Green
MitoROS 520
NBD
Neuro DiO
NL 493
NovaFluor Blue 530
NovaFluor Blue 610 / 30S
Nuclear Green
Nuclear Orange
NucSpot 470
NucSpot Live 488
Oxazole Yellow
Oxazole Yellow Homodimer
Oyster 488
PE
PE-ATTO 594
PE-CF594
PE-Dazzle 594
PE-DyLight 594
PE-Dyomics 590
PE-eFluor 610
PE-Vio615
PhenoVue 493 Lipid Stain
PhenoVue 503 Lysosomal Stain
PhenoVue 512 Nucleic Acid Stain
PhenoVue Cal-520 AM Bright, Calcium Indicator
PhenoVue Cal-520 AM, Calcium Indicator
PhenoVue Fluo-4 AM, Calcium Indicator
PhenoVue Fluor 488
PhenoVue ROS-490, Total Oxidative Stress Indicator
PicoGreen
PromoFluor 488P
RealBlue 545
SNARF 1 pH6.0
Spark Blue 550
Spark Blue 574
Spectrum Green
StarBright Blue 580
StarBright Blue 615
SureLight 488
SureLight PE-594
Syto 16
Syto 24
Thiazole Orange Homodimer
Thiazole Orange Iodide
Vio 488
Vio 515
Vio Bright 515
Vio Bright FITC
VRDye 490

530 / 30

--

502

 Alexa Fluor 488
FITC
GFP
MitoTracker Green
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

585 / 42

--

556

 dsRed
PE
Propidium Iodide

--

--

670

 PerCP
PerCP-Cy5.5
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

--

735

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

--

 Alexa Fluor 647
APC
MitoTracker Deep Red
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650

780 / 60

--

735

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.