University of Vermont
Harry Hood Bassett Flow Cytometry and Cell Sorting Facility

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LSR II - BUV 395/737 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395

740 / 35

--

 Brilliant Ultraviolet 737
Qdot 705
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
Cascade Blue
eFluor 450
Pacific Blue
V450
Vio Blue
CF405M
DyLight 405
eBFP

525 / 50

505

 Alexa Fluor 430
Brilliant Violet 510
Pacific Orange
V500
Vio Green
AmCyan
Cascade Yellow
CF405L
CF430
CF440

610 / 20

--

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600
Laser

488

530 / 30

505

 Alexa Fluor 488
CFSE
eGFP
FITC
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

575 / 26

550

 PE

610 / 20

600

 ECD
PE-Alexa 610
PE-CF594
PE-Texas Red
Propidium Iodide
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

695 / 40

685

 PE-Cy5.5
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
7-AAD
Brilliant Blue 700
PerCP-Vio700

780 / 60

735

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 660
Alexa Fluor 680
APC
eFluor 660
Alexa Fluor 647
APC-Cy5.5
CF640R
CF647
Cy5
DyLight 633

730 / 45

710

 Alexa Fluor 700
APC-R700

780 / 60

755

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Vio770
APC-Fire 750

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.