University of Vermont
Harry Hood Bassett Flow Cytometry and Cell Sorting Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/uvm
Logo: FluoroFinder

LSR II - Indo-1 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

405 / 20

--

 Indo-Hi (Ca2+bound)

530 / 30

505

 Indo-Lo (Ca2+free)
Laser

488

530 / 30

505

 Alexa Fluor 488
CFSE
eGFP
FITC
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

575 / 26

550

 PE

610 / 20

600

 ECD
PE-Alexa 610
PE-CF594
PE-Texas Red
Propidium Iodide
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

695 / 40

685

 PE-Cy5.5
PerCP
PerCP-Cy5.5
PerCP-eFluor 710
7-AAD
Brilliant Blue 700
PerCP-Vio700

780 / 60

735

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 633
Alexa Fluor 647
APC
eFluor 660
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650

--

710

 Alexa Fluor 700
APC-Cy5.5
Alexa Fluor 680
APC-R700
DyLight 680

780 / 60

755

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Vio770
APC-Fire 750

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.