University of Virginia (UVA)
VA School of Medicine - FCCF

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LSRFortessa Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
DAPI
Live/Dead Fix Violet
Pacific Blue
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450

525 / 50

505

 AmCyan
Brilliant Violet 510
Live/Dead Fix Yellow
Qdot 525
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500

610 / 20

595

 Brilliant Violet 605
Pacific Orange
Qdot 605
Qdot 625
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 20

630

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 785
Qdot 800
Laser

488

525 / 50

505

 Alexa Fluor 488
Brilliant Blue 515
FITC
GFP
YFP
ATTO 488
CF488A
CF503R
Cy2
DyLight 488

670 / 14

600

 PerCP

695 / 40

690

 PerCP-Cy5.5
PerCP-eFluor 710
Brilliant Blue 700
PerCP-Vio700
Laser

561

582 / 14

565

 dsRed
PE
CF550R
CF568
CF570

610 / 20

600

 Alexa Fluor 568
Alexa Fluor 610
mCherry
PE-Texas Red
CF583
CF583R
Cy3.5
DyLight 594
Dyomics 590
ECD

670 / 30

640

 PE-Cy5
7-AAD

710 / 50

685

 PE-Cy5.5

780 / 60

735

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

710 / 50

685

 Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

750

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Vio770
APC-Fire 750

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.