University of Virginia (UVA)
VA School of Medicine - FCCF

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BD Influx Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

460 / 50

--

 Brilliant Ultraviolet 496
DAPI
Pacific Blue
DyLight 350
DyLight 405

585 / 15

--

 Brilliant Ultraviolet 563

740 / 35

--

 Brilliant Ultraviolet 737
Laser

405

460 / 50

--

 Brilliant Violet 421
Brilliant Violet 480
DAPI
Pacific Blue
SuperBright 436
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
V450

520 / 35

--

 Brilliant Violet 510
Live/Dead Fix Aqua
Qdot 525
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

610 / 20

--

 Brilliant Violet 605
SuperBright 600
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605

660 / 20

--

 Brilliant Violet 650
SuperBright 645
eFluor 650NC
eVolve 655
Qdot 655

710 / 20

--

 Brilliant Violet 711
SuperBright 702
Qdot 705

--

750

 Brilliant Violet 750
Brilliant Violet 785
Qdot 800
Laser

488

530 / 40

--

 Alexa Fluor 488
Brilliant Blue 515
CFSE
FITC
GFP
Sytox Green
YFP
ATTO 488
CF488A
CF503R

716 / 40

--

 Brilliant Blue 700
PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
Laser

561

585 / 29

--

 dsRed
PE
PKH26
RFP
CF550R
CF568
CF570

610 / 20

--

 Alexa Fluor 568
Live/Dead Fix Red
mCherry
PE-Alexa 610
PE-CF594
PE-Texas Red
CF583
CF583R
Cy3.5
DyLight 594

670 / 30

--

 PE-Alexa 647
PE-Cy5
7-AAD

--

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 647
APC
eFluor 660
Live/Dead Fix Far Red
TO-Pro 3
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5

720 / 40

--

 Alexa Fluor 700
APC-Cy5.5
APC-R700
Spark NIR 685
Alexa Fluor 680
DyLight 680

--

750

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
Live/Dead Fix Near IR (775)
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.