University of Virginia (UVA)
VA School of Medicine - FCCF

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FACSVantage SE DIVA Configuration

Laser (nm) BP SP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 20

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 Alexa Fluor 405
DAPI
eFluor 450
Live/Dead Fix Violet
Pacific Blue
CF405M
DyLight 405
V450
Vio Blue

510 / 20

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 AmCyan
Brilliant Violet 510
Live/Dead Fix Yellow
Qdot 525
Alexa Fluor 430
CF405L
CF430
CF440
V500
Vio Green
Laser

488

530 / 30

560

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 Alexa Fluor 488
Cy2
DyLight 488
FITC
Fluorescein
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A

585 / 42

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 PE

695 / 40

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 PE-Cy5.5
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

787 / 42

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 PE-Cy7
PE-Vio770
Laser

592

660 / 20

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 APC

780 / 60

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 APC-Cy7
Laser

640

660 / 20

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 Alexa Fluor 647
APC
Cy5
DyLight 633
DyLight 650
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE

780 / 60

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 APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.