University of Virginia (UVA)
VA School of Medicine - FCCF

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ImageStreamX Mark II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

462 / 85

--

 Alexa Fluor 405
Brilliant Violet 421
DAPI
eFluor 450
Pacific Blue
V450
CF405M
DyLight 405
SuperBright 436
Vio Blue

537 / 65

--

 Alexa Fluor 430
Brilliant Violet 510
Pacific Orange
V500
Brilliant Violet 570
CF405L
CF430
CF440
DyLight 405LS
Krome Orange

582 / 25

--

 Qdot 585
Brilliant Violet 570

627 / 65

--

 Brilliant Violet 605
eFluor 605NC
Qdot 625
DyLight 405LS
eVolve 605
Qdot 605
SuperBright 600

700 / 80

--

 Brilliant Violet 711
eFluor 650NC
Qdot 705
Brilliant Violet 650
eVolve 655
Qdot 655
SuperBright 702

770 / 60

--

 Brilliant Violet 785
Qdot 800
Brilliant Violet 786
Laser

488

520 / 40

--

 Alexa Fluor 488
Brilliant Blue 515
DyLight 488
FITC
GFP
YFP
ATTO 488
CF488A
CF503R
Cy2

577 / 35

--

 dsRed
PE

627 / 65

--

 ECD
PE-Texas Red
Propidium Iodide
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

700 / 80

--

 PE-Cy5
PE-Cy5.5
PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

770 / 60

--

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

561

577 / 35

--

 dsRed
DyLight 549
PE
CF550R
CF568
CF570

627 / 65

--

 Alexa Fluor 594
DyLight 594
ECD
mCherry
PE-Texas Red
Propidium Iodide
RFP
Texas Red
BODIPY TR
CF583

700 / 80

--

 PE-Cy5
PE-Cy5.5

770 / 60

--

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

640

700 / 80

--

 Alexa Fluor 647
APC
APC-Cy5.5
DyLight 649
Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 680
CF640R
CF647
Cy5

770 / 60

--

 APC-Cy7
APC-eFluor 780
APC-H7
DyLight 750
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.