University of Virginia (UVA)
VA School of Medicine - FCCF

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BD Aria Fusion Cell Sorter (488, 561, 640, 375 lasers) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

375

450 / 50

--

 DAPI
Hoechst (33258)
Live/Dead Fix Blue

525 / 50

505

 Qdot 525

610 / 20

595

 Hoechst (33342)
Qdot 605
Qdot 625
Brilliant Violet 605
eVolve 605

660 / 20

630

 Qdot 655
eVolve 655

710 / 50

690

 Qdot 705

780 / 60

750

 Qdot 800
Brilliant Violet 786
Laser

488

530 / 30

502

 Alexa Fluor 488
Brilliant Blue 515
eGFP
FITC
Sytox Green
ATTO 488
CF488A
CF503R
Cy2
DyLight 488

695 / 40

655

 PerCP-Cy5.5
PerCP-eFluor 710
Brilliant Blue 700
PerCP-Vio700
Laser

561

582 / 14

--

 dsRed
PE
CF550R
CF568
CF570

610 / 20

600

 Alexa Fluor 568
mCherry
PE-Alexa 610
PE-CF594
PE-Dazzle 594
PE-Texas Red
CF583
CF583R
Cy3.5
DyLight 594

670 / 14

655

 PE-Cy5
PE-Cy5.5
7-AAD

780 / 60

735

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
Dyomics 647

780 / 60

735

 APC-Cy7
APC-eFluor 780
APC-H7
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.