University of Virginia (UVA)
VA School of Medicine - FCCF

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BD/Cytek FACSCalibur B (Blue/Violet) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

407

440 / 50

--

 CFP
DAPI
Pacific Blue
V450
Alexa Fluor 405
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
Vio Blue

545 / 30

--

 Pacific Orange
V500
Alexa Fluor 430
Brilliant Violet 510
Brilliant Violet 570
DyLight 405LS
Krome Orange
Vio Green
Laser

488

530 / 30

--

 Alexa Fluor 488
CFSE
FITC
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

585 / 42

--

 PE
Propidium Iodide

--

670

 PE-Cy5
PE-Cy5.5
PerCP
PerCP-Vio700

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.