University of Virginia (UVA)
VA School of Medicine - FCCF

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BD/Cytek Calibur A Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

407

450 / 50

--

 Alexa Fluor 405
CFP
DAPI
Pacific Blue
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

545 / 30

--

 AmCyan
Brilliant Violet 570
Pacific Orange
Qdot 545
Alexa Fluor 430
Brilliant Violet 510
DyLight 405LS
Krome Orange
Qdot 565
V500
Laser

488

530 / 30

--

 Alexa Fluor 488
CFSE
FITC
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

695 / 40

--

 PE-Cy5.5
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

580 / 20

--

 dsRed
PE
CF550R
CF568
CF570

620 / 20

--

 Alexa Fluor 568
mCherry
PE-Alexa 610
PE-Texas Red
CF583
CF583R
Cy3.5
DyLight 594
Dyomics 590
ECD

--

740

 PE-Cy7
PE-Vio770
Laser

637

661 / 16

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
NL 637

710 / 20

--

 Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

--

740

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.