University of Pittsburgh
UPMC Hillman Cancer Center Cytometry Facility

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(Hillman) - Astrios Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

448 / 59

--

 Hoechst (33258)

692 / 75

--

 Hoechst (33342)
Laser

405

448 / 59

--

 Brilliant Violet 421
DAPI
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

530 / 40

--

 Brilliant Violet 510
Live/Dead Fix Aqua
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

710 / 45

--

 Brilliant Violet 711
Qdot 705
SuperBright 702
Laser

488

526 / 52

--

 FITC
GFP
Vio Bright FITC
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

576 / 21

--

 dsRed
PE
Sytox Orange

692 / 75

--

 PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

579 / 16

--

 dsRed
PE
Sytox Orange
CF550R
CF568
CF570

620 / 29

--

 mCherry
PE-CF594
PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

664 / 22

--

 PE-Cy5
PE-Dyomics 647

710 / 45

--

 PE-Cy5.5

795 / 70

--

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

640

670 / 30

--

 Alexa Fluor 647
APC
eFluor 660
TO-Pro 3
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633

722 / 44

--

 Alexa Fluor 700
APC-Alexa 700
APC-R700

780 / 60

--

 APC-Cy7
APC-Vio770
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.