University of Pittsburgh
UPMC Hillman Cancer Center Cytometry Facility

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(Assembly) - Thermo Bigfoot Cell Sorter (Spectral) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

349

387 / 11

--

420 / 10

--

434 / 17

--

455 / 14

--

473 / 15

--

507 / 19

--

549 / 15

--

575 / 15

--

615 / 24

--

670 / 30

--

728 / 40

--

--

750
Laser

405

420 / 10

--

434 / 17

--

455 / 14

--

473 / 15

--

507 / 19

--

549 / 15

--

575 / 15

--

615 / 24

--

661 / 20

--

710 / 20

--

747 / 33

--

--

770
Laser

488

507 / 19

--

549 / 15

--

583 / 30

--

615 / 24

--

670 / 30

--

720 / 60

--

--

750
Laser

561

575 / 15

--

589 / 15

--

605 / 15

--

625 / 15

--

661 / 20

--

685 / 15

--

700 / 13

--

720 / 24

--

760 / 50

--

800 / 12

--

832 / 37

--

--

860
Laser

640

670 / 30

--

700 / 13

--

720 / 24

--

760 / 50

--

--

770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.