University of Pittsburgh
UPMC Hillman Cancer Center Cytometry Facility

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(Hillman) - CytoFlex 2L Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

488

525 / 40

--

 Adirondack Green 520
Alexa Fluor 488
Alpha Fluor 488
ATTO 488
BactoView Live Green
BCECF AM
Brilliant Blue 515
CellTrace Oregon Green
CF488A
CF500
cFluor B515
cFluor B532
cFluor B548
Chromeo 488
Chromeo 505
CoraLite 532
CoraLite Plus 488
CytoTrace Green CMFDA
DAF-FM
DMAO
DyLight 488
ExoBrite 490/515 EV Membrane Stain
FITC
FITC Plus
HiLyte 488
iFluor 488
KIRAVIA Blue 520
LysoBrite Green
LysoView 488
MaxLight 490
MemBrite Fix 488/515
MitoLite Green EX488
MitoLite Green FM
MitoMark Green
MitoROS 520
NBD
Neuro DiO
NL 493
NovaFluor Blue 530
Nuclear Green
Nuclear Orange
NucSpot 470
NucSpot Live 488
Oxazole Yellow
Oxazole Yellow Homodimer
Oyster 488
PhenoVue 493 Lipid Stain
PhenoVue 503 Lysosomal Stain
PhenoVue Cal-520 AM Bright, Calcium Indicator
PhenoVue Cal-520 AM, Calcium Indicator
PhenoVue Fluo-4 AM, Calcium Indicator
PhenoVue Fluor 488
PhenoVue ROS-490, Total Oxidative Stress Indicator
PicoGreen
PromoFluor 488P
RealBlue 545
Spark Blue 550
Spark Blue 574
Spectrum Green
SureLight 488
Syto 16
Syto 24
Thiazole Orange Homodimer
Thiazole Orange Iodide
Vio 488
Vio 515
Vio Bright 515
Vio Bright FITC
VRDye 490

585 / 42

--

 Alexa Fluor 555
cFluor B548
cFluor BYG575
cFluor BYG610
Chromeo 494
CoraLite 532
Di-8-ANEPPS
NBD
NovaFluor Blue 610 / 30S
Nuclear Green
Nuclear Orange
NucSpot 470
PE
PE-CF594
PE-Dazzle 594
PE-DyLight 594
PE-Dyomics 590
PE-eFluor 610
PE-Vio615
PhenoVue 512 Nucleic Acid Stain
RealBlue 545
SNARF 1 pH6.0
Spark Blue 574
StarBright Blue 580
StarBright Blue 615
SureLight PE-594
Thiazole Orange Homodimer
Thiazole Orange Iodide

690 / 50

--

 ATTO 490LS
Brilliant Blue 700
cFluor B690
cFluor BYG667
cFluor BYG680
cFluor BYG710
Chromeo 494
Di-8-ANEPPS
NovaFluor Blue 660 / 40S
NovaFluor Blue 690
PE-Cy5
PE-Cy5.5
PE-Dyomics 647
PE-Fire 700
PerCP-eFluor 710
PerCP-Vio700
PE-Vio670
RealBlue 705
RPE-CF647T
StarBright Blue 675
StarBright Blue 700
SureLight PE Cyanine 5.0
SureLight PE Cyanine 5.5
Laser

638

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
Alexa Fluor 660
APC
ATTO 633
ATTO 647
ATTO 647N
ATTO 655
ATTO 665
Calcein Deep Red AM
CellMask Deep Red Plasma Membrane Stain
CellTracker Deep Red
CF633
CF640R
CF647
cFluor R685
Chromeo 642
CoraLite 647A
CoraLite Plus 647
Cy5
CypHer5E
DiIC1(5)
DRAQ 9
DyLight 633
DyLight 649
DyLight 650
ExoBrite 640/660 EV Membrane Stain
FluoroProbes647h
HiLyte 647
iFluor 633
iFluor 647
IRDye 650
Janelia Fluor 635
Janelia Fluor 646
LipidSpot 610
LysoBrite NIR
LysoView 640
LysoView 650
MaxLight 650
MemBrite Fix 640/660
MemBrite Fix ST 650/665
MitoLite Deep Red FX660
MitoLite NIR FX690
MitoView 650
MitoView Fix 640
NL 637
NucSpot 640
NucSpot Far-Red
NucSpot Live 650
Oyster 650
PhenoVue 641 Mitochondrial Stain
PhenoVue Fluor 647
PhenoVue ROS-670, Total Oxidative Stress Indicator
PromoFluor 647P
Spark NIR 685
STAR 635
STAR 635P
STAR RED
SureLight APC
SureLight P3
SureLight RPC
Thiazole Red
Thiazole Red Homodimer
Vio 667
Vio Bright 667
Vio Bright R667
Vio R667

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.