University of Pittsburgh
UPMC Hillman Cancer Center Cytometry Facility

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(Hillman) - Fortessa II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395
Indo-Hi (Ca2+bound)

670 / 25

--

 Brilliant Ultraviolet 661
eVolve 655
Qdot 655
eFluor 650NC

740 / 35

--

 Brilliant Ultraviolet 737
Qdot 705
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
V450
Vio Blue
Cascade Blue
CF405M
DyLight 405
eBFP
eFluor 450
Pacific Blue

515 / 20

--

 Alexa Fluor 430
Brilliant Violet 480
Vio Green
AmCyan
Brilliant Violet 510
Cascade Yellow
Krome Orange
Pacific Green
Pacific Orange
Qdot 525

670 / 30

--

 Brilliant Violet 650
eVolve 655
Qdot 655
eFluor 650NC
SuperBright 645

710 / 45

--

 Brilliant Violet 711
Brilliant Violet 750
Qdot 705
SuperBright 702

780 / 60

--

 Brilliant Violet 785
Qdot 800
Brilliant Violet 786
Laser

488

515 / 30

--

 Brilliant Blue 515
FITC
GFP
Vio Bright FITC
Alexa Fluor 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

710 / 50

--

 PerCP-Cy5.5
PerCP-eFluor 710
PerCP-Vio700
Brilliant Blue 700
PE-Cy5
PE-Cy5.5
Laser

561

582 / 15

--

 dsRed
DyLight 550
PE
CF550R
CF568
CF570

610 / 20

--

 Live/Dead Fix Red
PE-Dazzle 594
DyLight 594
ECD
PE-CF594
PE-Texas Red
PE-Vio615

660 / 40

--

 PE-Alexa 647
PE-Cy5
7-AAD

710 / 50

--

 DRAQ 7
PE-Alexa 700

780 / 40

--

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

628

670 / 30

--

 Alexa Fluor 647
APC
Cy5
Alexa Fluor 633
Alexa Fluor 635
CF640R
Cyanine 5 SE
DyLight 633
DyLight 650
NL 637

730 / 45

--

 Alexa Fluor 700
APC-R700

780 / 60

--

 APC-CF750
APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.