University of Nebraska Med Center
UNMC Flow Cytometry Research Facility

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BD FACSAria 2 (14color) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 Alexa Fluor 350
DAPI
Hoechst (33258)
Indo-Hi (Ca2+bound)
Live/Dead Fix Blue
CF350
DyLight 350

525 / 50

505

 Hoechst (33342)
Indo-Lo (Ca2+free)
Laser

405

450 / 50

--

 eFluor 450
Pacific Blue
SuperBright 436
V450
Alexa Fluor 405
Brilliant Violet 421
CF405M
DyLight 405
Vio Blue

525 / 50

--

 AmCyan
Pacific Orange
Qdot 525
V500
Alexa Fluor 430
Brilliant Violet 510
DyLight 405LS
Krome Orange
Vio Green

605 / 40

--

 Brilliant Violet 605
SuperBright 600
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
Laser

488

530 / 30

--

 Alexa Fluor 488
CFSE
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

575 / 26

--

 PE
YFP

610 / 20

--

 ECD
PE-Texas Red
Propidium Iodide
7-AAD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

660 / 20

--

 PE-Cy5
PerCP-Vio700

710 / 40

--

 PE-Cy5.5
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

--

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 647
APC
Cy5
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

710 / 50

--

 Alexa Fluor 700
APC-Cy5.5
Alexa Fluor 680
APC-R700
DyLight 680

780 / 60

--

 APC-Cy7
APC-eFluor 780
APC-H7
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.