University of Nebraska Med Center
UNMC Flow Cytometry Research Facility

Design a panel and find antibodies today at

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BD FACSAria (14color) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

Brilliant Ultraviolet 395

450 / 50

--

Alexa Fluor 350
DAPI
Hoechst (33258)
Live/Dead Fix Blue
CF350
DyLight 350
Laser

405

450 / 50

--

Brilliant Violet 421
eFluor 450
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
SuperBright 436
Vio Blue

520 / 30

--

AmCyan
Brilliant Violet 510
Qdot 525
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

610 / 20

--

Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600
Laser

488

530 / 30

--

Alexa Fluor 488
CFSE
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

695 / 40

--

PE-Cy5
PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

582 / 15

--

Cy3
dsRed
PE
tdTomato
Alexa Fluor 546
Alexa Fluor 555
CF550R
CF568
CF570
Cyanine 3 SE

605 / 15

--

Alexa Fluor 568
Alexa Fluor 594
Alexa Fluor 610
mCherry
PE-Texas Red
7-AAD
BODIPY TR
CF583
CF583R
Cy3.5

660 / 40

--

PE-Cy5
PE-Cy5.5

780 / 60

--

PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

Alexa Fluor 633
Alexa Fluor 647
APC
Cy5
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

710 / 50

--

Alexa Fluor 700
APC-R700

780 / 60

--

APC-Cy7
APC-eFluor 780
APC-H7
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.