University of Nebraska Med Center
UNMC Flow Cytometry Research Facility

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BD Fortessa X-50 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

Brilliant Ultraviolet 395
Indo-Hi (Ca2+bound)

515 / 30

--

Brilliant Violet 510
DAPI
Hoechst (33258)
Indo-Lo (Ca2+free)

586 / 15

--

eFluor 605NC
Hoechst (33342)
Qdot 585
eVolve 605

615 / 24

--

eFluor 625NC
Qdot 605
eVolve 605

660 / 20

--

Qdot 655
eVolve 655

740 / 35

--

Brilliant Ultraviolet 737
Qdot 705

820 / 60

--

Brilliant Ultraviolet 805
Qdot 800
Laser

405

427 / 25

--

Alexa Fluor 405
Brilliant Violet 421
eFluor 450
DyLight 405
SuperBright 436
V450
Vio Blue

525 / 50

--

Brilliant Violet 510
eCFP
Qdot 545
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

586 / 15

--

Brilliant Violet 605
Qdot 585
Brilliant Violet 570
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

610 / 20

--

eFluor 605NC
Pacific Orange
Qdot 625
SuperBright 600
DyLight 405LS
eVolve 605

670 / 30

--

Brilliant Violet 650
eFluor 650NC
Qdot 655
eVolve 655
SuperBright 645

710 / 40

--

Brilliant Violet 711
Qdot 705
SuperBright 702

750 / 30

--

Brilliant Violet 785
Qdot 800
Brilliant Violet 786

780 / 60

--

Brilliant Violet 786
Qdot 800
Laser

488

525 / 50

--

Alexa Fluor 514
Brilliant Blue 515
Brilliant Violet 480
Alexa Fluor 488
CF488A
CF503R
DyLight 488
FITC
Vio Bright FITC

586 / 15

--

PE
Propidium Iodide

610 / 20

--

ECD
PE-Dazzle 594
PE-CF594
PE-Texas Red
PE-Vio615

710 / 50

--

PE-Cy5.5
PerCP
PerCP-Cy5.5
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

--

LDS 751
PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

561

586 / 15

--

Alexa Fluor 546
Cy3
dsRed
eFluor 570
Alexa Fluor 555
ATTO 550
Cyanine 3 SE
DyLight 549
DyLight 550
Dyomics 547

610 / 20

--

Alexa Fluor 594
PE-Alexa 610
PE-CF594
BODIPY TR
DyLight 594
ECD
PE-Dazzle 594
PE-Texas Red
PE-Vio615
Texas Red

670 / 30

--

PE-Alexa 647
PE-Cy5
PE-Cy5.5

710 / 25

--

DRAQ 7
PE-Alexa 700
7-AAD

780 / 60

--

LDS 751
PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

633

670 / 30

--

Alexa Fluor 647
APC
Cy5
eFluor 660
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633

730 / 45

--

Alexa Fluor 700
APC-Alexa 680
Cy5.5
APC-R700

780 / 60

--

APC-Alexa 750
APC-Cy7
APC-H7
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.