University of Lausanne
Ludwig Center for Cancer Research Flow Cytometry Facility

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Canto (405/488/633) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Brilliant Violet 421
CFP
DAPI
eFluor 450
Alexa Fluor 405
CF405M
DyLight 405
Pacific Blue
SuperBright 436
V450

525 / 50

505

 Brilliant Violet 510
Live/Dead Fix Aqua
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

610 / 20

595

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600
Laser

488

525 / 50

502

 Alexa Fluor 488
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

575 / 26

556

 PE

610 / 20

600

 ECD
PE-CF594
PE-eFluor 610
PE-Texas Red
PE-Dazzle 594
PE-Vio615

660 / 20

655

 PE-Cy5
7-AAD
PerCP-Vio700

710 / 40

685

 PE-Cy5.5
PerCP-Cy5.5
PerCP-eFluor 710
Brilliant Blue 700
PerCP-Vio700

780 / 60

755

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 647
APC
eFluor 660
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650

710 / 50

685

 Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

755

 APC-Cy7
APC-eFluor 780
APC-H7
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.