University of Lausanne
Ludwig Center for Cancer Research Flow Cytometry Facility

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LSR IIa (405/488/561/633) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Brilliant Violet 421
CFP
DAPI
eFluor 450
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
SuperBright 436
V450

515 / 20

495

 Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
V500
Vio Green

585 / 42

570

 Brilliant Violet 570
Krome Orange
Live/Dead Fix Aqua
Pacific Orange
Qdot 565
Alexa Fluor 430
DyLight 405LS
Qdot 585

610 / 20

600

 Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 20

635

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 786
Qdot 800
Brilliant Violet 785
Laser

488

530 / 50

505

 Alexa Fluor 488
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

695 / 40

670

 PerCP-Cy5.5
PerCP-eFluor 710
Brilliant Blue 700
PerCP-Vio700
Laser

561

582 / 15

--

 PE
RFP
CF550R
CF568
CF570

610 / 20

600

 ECD
mCherry
PE-CF594
PE-eFluor 610
PE-Texas Red
CF583
CF583R
DyLight 594
PE-Dazzle 594
PE-Vio615

670 / 14

655

 PE-Cy5

695 / 40

685

 PE-Cy5.5

780 / 60

735

 PE-Cy7
PE-Vio770
Laser

633

670 / 14

--

 Alexa Fluor 647
APC
eFluor 660
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650

730 / 45

685

 Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

735

 APC-Cy7
APC-eFluor 780
APC-H7
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.