University of North Carolina (UNC)
UNC Flow Cytometry Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/unc-ch
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ImageStreamX Mark II Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

462 / 85

--

 Alexa Fluor 405
Brilliant Violet 421
Cascade Blue
CFP
DAPI
eFluor 450
Live/Dead Fix Violet
Marina Blue
Pacific Blue
Spectrum Aqua
V450

537 / 65

--

 Alexa Fluor 430
Brilliant Violet 510
Cascade Yellow
Live/Dead Fix Aqua
Lucifer Yellow
Pacific Orange
Qdot 525
Qdot 545
V500
Brilliant Violet 570

582 / 25

--

 Qdot 565
Qdot 585
Brilliant Violet 570

627 / 65

--

 eFluor 605NC
Qdot 605
Qdot 625
SuperBright 600
SuperBright 645
Brilliant Violet 605
DyLight 405LS
eVolve 605

700 / 80

--

 eFluor 650NC
Qdot 705
SuperBright 702
Brilliant Violet 650
eVolve 655
Qdot 655

770 / 60

--

 Qdot 800
Brilliant Violet 785
Brilliant Violet 786
Laser

488

520 / 40

--

 Alexa Fluor 488
Alexa Fluor 514
Calcium Green
DyLight 488
FITC
GFP
LysoTracker Green
MitoTracker Green
Spectrum Green
Vybrant DyeCycle Green
YFP

577 / 35

--

 Cy3
DiI
dsRed
OFP
PE
Alexa Fluor 555
Cyanine 3 SE

627 / 65

--

 ECD
PE-Alexa 610
PE-Texas Red
Propidium Iodide
7-AAD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

700 / 80

--

 DRAQ 5
PE-Alexa 647
PE-Cy5
PE-Cy5.5
PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

770 / 60

--

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

658

700 / 80

--

 Alexa Fluor 647
Alexa Fluor 660
Alexa Fluor 680
APC
APC-Cy5.5
Cy5
Cy5.5
DRAQ 5
DyLight 649
MitoTracker Deep Red
Nile Blue
TO-Pro 3

770 / 60

--

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
DyLight 750
APC-H7

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.