University of North Carolina (UNC)
UNC Flow Cytometry Facility

Design a panel and find antibodies today at

https://app.fluorofinder.com/unc-ch
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Attune NXT Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 40

417

Brilliant Violet 421
DAPI
eFluor 450
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
SuperBright 436
V450
Vio Blue

525 / 50

495

ATTO 425
Brilliant Violet 510
eFluor 506
Pacific Green
Alexa Fluor 430
AmCyan
CF405L
CF430
CF440
DY-380XL

610 / 20

555

Brilliant Violet 605
Pacific Orange
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

660 / 20

635

Brilliant Violet 650
SuperBright 645
eFluor 650NC
eVolve 655
Qdot 655

710 / 50

680

Brilliant Violet 711
SuperBright 702
Qdot 705

780 / 60

740

Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

503

Alexa Fluor 488
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

695 / 40

555

PerCP
PerCP-Cy5.5
PerCP-eFluor 710
7-AAD
Brilliant Blue 700
PerCP-Vio700
Laser

561

585 / 16

577

dsRed
dTomato
PE
CF550R
CF568
CF570

620 / 15

600

Alexa Fluor 594
PE-Texas Red
Propidium Iodide
7-AAD
BODIPY TR
CF583
CF583R
DyLight 594
ECD
PE-CF594

780 / 60

650

PE-Cy7
PE-Vio770
Laser

637

670 / 14

654

Alexa Fluor 647
APC
eFluor 660
Sytox Red
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633

720 / 30

690

Alexa Fluor 700
APC-R700

780 / 60

740

APC-Alexa 750
APC-Cy7
APC-eFluor 780
Vybrant DyeCycle Ruby
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.