University of Michigan
Biomedical Research Flow Cytometry Core

Design a panel and find antibodies today at

https://app.fluorofinder.com/umich
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Bio-Rad Ze5 Analyzer 4-laser (405, 488, 561, 640nm lasers) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

420 / 10

--

 Brilliant Violet 421
Alexa Fluor 405
DyLight 405
SuperBright 436
Vio Blue

460 / 22

--

 Brilliant Violet 480
Pacific Blue
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
V450
Vio Blue

525 / 50

--

 Brilliant Violet 510
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

615 / 24

--

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 30

--

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

720 / 60

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

--

750

 Brilliant Violet 750
Brilliant Violet 786
Qdot 800
Laser

488

525 / 35

--

 Alexa Fluor 488
FITC
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

593 / 52

--

 PE

692 / 80

--

 PE-Cy5
PerCP-Vio700

--

750

 PE-Cy7
PE-Vio770
Laser

561

577 / 15

--

 PE
CF550R
CF568
CF570

589 / 15

--

 dsRed

615 / 24

--

 mCherry
Propidium Iodide

640 / 20

--

 PE-Cy7

670 / 30

--

 PE-Cy5

720 / 60

--

 PE-Cy7

--

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 PE-Cy7

720 / 60

--

 Alexa Fluor 647
APC
Dyomics 647

775 / 50

--

 Alexa Fluor 680
Alexa Fluor 700

--

800

 Alexa Fluor 750
APC-Cy7
APC-Alexa 750
APC-Fire 750
APC-H7

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.