University of Michigan
Biomedical Research Flow Cytometry Core

Design a panel and find antibodies today at

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Amnis Imagestream Mark II Single-Camera system 4-laser (405, 488, 561, 640nm lasers) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

457 / 44

--

 Brilliant Violet 421
DAPI
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
Pacific Blue
V450
Vio Blue

533 / 54

--

 Alexa Fluor 350
Brilliant Violet 510
Cascade Yellow
Pacific Orange
Alexa Fluor 430
Brilliant Violet 570
CF405L
CF430
CF440
DyLight 405LS

577 / 34

--

 Qdot 585
Brilliant Violet 570

610 / 30

--

 Brilliant Violet 605
Qdot 625
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

702 / 84

--

 Brilliant Violet 711
Qdot 705
SuperBright 702

762 / 34

--

 Brilliant Violet 785
Qdot 800
Brilliant Violet 786
Laser

488

457 / 44

--

 DAPI

533 / 54

--

 Alexa Fluor 488
FITC
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

577 / 34

--

 PE

610 / 30

--

 ECD
PE-Texas Red
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

702 / 84

--

 PE-Alexa 647
PE-Cy5
PerCP-Vio700

762 / 34

--

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

561

577 / 34

--

 Alexa Fluor 546
PE
Alexa Fluor 555
ATTO 550
CF550R
CF568
CF570
Cy3
DyLight 549
DyLight 550

610 / 30

--

 Alexa Fluor 568
PE-Texas Red
CF583
CF583R
Cy3.5
DyLight 594
Dyomics 590
ECD
PE-CF594
PE-Dazzle 594

702 / 84

--

 PE-Cy5
7-AAD

762 / 34

--

 PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

640

702 / 84

--

 Alexa Fluor 647
Alexa Fluor 660
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650

762 / 34

--

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.