University of Groningen (UMCG)
UMCG Flow Cytometry Department

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BD Symphony A50 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

370

 Brilliant Ultraviolet 395

450 / 50

410

 Alexa Fluor 350
DAPI
CF350
DyLight 350

515 / 30

490

 Brilliant Ultraviolet 496

578 / 21

550

 Brilliant Ultraviolet 563

614 / 20

600

 Qdot 605
Qdot 625
eVolve 605

670 / 20

630

 Brilliant Ultraviolet 661

735 / 30

690

 Brilliant Ultraviolet 737

810 / 40

791

 Brilliant Ultraviolet 805
Laser

405

431 / 28

410

 Brilliant Violet 421
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
Pacific Blue
SuperBright 436
V450
Vio Blue

525 / 50

505

 Brilliant Violet 480

578 / 21

550

 Brilliant Violet 570
DyLight 405LS
Qdot 565
Qdot 585

615 / 20

600

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

677 / 20

635

 Brilliant Violet 650
eFluor 650NC
eVolve 655

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

750 / 30

735

 Brilliant Violet 750

810 / 40

790

 Brilliant Violet 786
Qdot 800
Laser

445

470 / 15

455

 Brilliant Violet 480
Cerulean
CFP
Laser

488

530 / 30

505

 Brilliant Blue 515
FITC
Alexa Fluor 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

615 / 20

600

 Brilliant Blue 630-P2

670 / 30

635

 Brilliant Blue 660-P2

710 / 50

685

 Brilliant Blue 700
PerCP-Cy5.5

750 / 30

735

 PE-Alexa 750
PE-Vio770

810 / 40

770

 PE-Cy7
PE-Vio770
Laser

561

578 / 21

570

 PE
CF550R
CF568
CF570

615 / 20

600

 PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 30

635

 PE-Cy5

710 / 50

685

 PE-Alexa 700

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

594

615 / 20

600

 Texas Red
Laser

637

670 / 30

655

 APC
Alexa Fluor 647
CF640R
CF647
Cy5
DyLight 633
NL 637

730 / 45

690

 APC-Alexa 700

780 / 60

750

 APC-Cy7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770
Laser

785

835 / 70

800

 Alexa Fluor 790
CF800

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.