University of Massachusetts Medical School
UMass Flow Cytometry Core Facility

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2 Aria (IIu) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

Brilliant Violet 421
Live/Dead Fix Violet
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
Vio Blue

530 / 30

502

AmCyan
Live/Dead Fix Aqua
Alexa Fluor 430
Brilliant Violet 510
V500
Vio Green

605 / 20

595

Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

655 / 20

630

Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

705 / 20

670

Brilliant Violet 711
Qdot 705
SuperBright 702

800 / 80

760

Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

502

Alexa Fluor 488
CFSE
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

695 / 40

655

PE-Cy5.5
PerCP-Cy5.5
PerCP-eFluor 710
7-AAD
Brilliant Blue 700
PerCP-Vio700
Laser

561

582 / 15

--

PE
tdTomato
CF550R
CF568
CF570

610 / 20

600

mCherry
PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 14

630

PE-Cy5
7-AAD

710 / 40

690

PE-Cy5.5

780 / 60

735

PE-Cy7
PE-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.