University of Massachusetts Medical School
UMass Flow Cytometry Core Facility

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Melody R6627500031 2B 2V 4YG Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

448 / 45

--

Brilliant Violet 421
DAPI
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
Vio Blue

528 / 45

500

AmCyan
Brilliant Violet 510
CFP
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green
Laser

488

527 / 32

507

Alexa Fluor 488
Brilliant Blue 515
FITC
ATTO 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
NL 493

700 / 54

665

Brilliant Blue 700
PerCP
PerCP-Cy5.5
7-AAD
PerCP-eFluor 710
PerCP-Vio700
Laser

561

582 / 15

582

dsRed
PE
tdTomato
CF550R
CF568
CF570

613 / 18

605

mCherry
PE-CF594
PE-Texas Red
CF583
CF583R
DyLight 594
ECD
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

697 / 58

665

PE-Cy5
PE-Cy5.5
7-AAD

783 / 56

752

PE-Cy7
PE-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.