University of Massachusetts Medical School
UMass Flow Cytometry Core Facility

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Aria Fusion "Figaro" (BSL-2+) 2B 6V 3R 2UV 4YG in BSC Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

410

 DAPI
Hoechst (33258)
Hoechst (33342)
Live/Dead Fix Blue
DyLight 350

670 / 50

635

 Brilliant Ultraviolet 661
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
eCFP
eFluor 450
Live/Dead Fix Violet
Pacific Blue
V450
CF405M
DyLight 405
SuperBright 436

525 / 50

505

 Brilliant Violet 510
Live/Dead Fix Aqua
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500
Vio Green

610 / 20

600

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600

670 / 30

635

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

710 / 50

690

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

750

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
CFSE
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

710 / 50

690

 PE-Cy5.5
PerCP-Cy5.5
PerCP-eFluor 710
7-AAD
Brilliant Blue 700
PerCP-Vio700
Laser

561

586 / 15

--

 Alexa Fluor 546
Cy3
dsRed
PE
PKH26
tdTomato
Alexa Fluor 555
ATTO 550
CF550R
CF568

610 / 20

600

 Alexa Fluor 594
Cy3
mCherry
PE-CF594
PE-Texas Red
Propidium Iodide
BODIPY TR
CF583
CF583R
Cyanine 3 SE

670 / 30

635

 PE-Cy5
Propidium Iodide
7-AAD

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 30

--

 Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
APC
Cy5
eFluor 660
CF640R
CF647
Cyanine 5 SE
DyLight 633

710 / 50

690

 Alexa Fluor 700
APC-Cy5.5
eFluor 710
Alexa Fluor 680
APC-R700
DyLight 680

780 / 60

750

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.