University of Massachusetts Medical School
UMass Flow Cytometry Core Facility

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BSL3 Aria (IIu) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 Alexa Fluor 350
CMA-Chromomycin A3
DAPI
Hoechst (33342)
Indo-Hi (Ca2+bound)
Live/Dead Fix Blue
CF350
DyLight 350

530 / 30

505

 Indo-Lo (Ca2+free)
Live/Dead Fix Aqua
Laser

405

450 / 50

--

 Alexa Fluor 405
eFluor 450
Live/Dead Fix Violet
Pacific Blue
V450
Brilliant Violet 421
CF405M
DyLight 405
Vio Blue

660 / 20

630

 Brilliant Violet 650
eFluor 650NC
eVolve 655
Qdot 655
SuperBright 645

780 / 60

750

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
CFSE
eGFP
FITC
GFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

575 / 24

550

 dsRed
PE

610 / 20

600

 ECD
EMA
PE-Texas Red
Propidium Iodide
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

710 / 50

685

 PE-Cy5
PE-Cy5.5
PerCP-Cy5.5
PerCP-eFluor 710
7-AAD
Brilliant Blue 700
PerCP-Vio700

780 / 60

755

 DRAQ 5
eFluor 615
PE-Alexa 750
PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 635
Alexa Fluor 647
APC
Cy5
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

685 / 34

675

 Alexa Fluor 700
APC-R700

730 / 44

690

 Alexa Fluor 700
APC-Alexa 700
APC-Cy5.5
eFluor 710
Alexa Fluor 680
APC-R700
DyLight 680

780 / 60

755

 APC-Alexa 750
APC-Cy7
APC-H7
APC-Vio770
APC-eFluor 780
APC-Fire 750

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.